Fig. 2

Bioassays for insecticide resistance to pyrethroids with Anopheles darlingi from the Brazilian Amazonian (2021–2024). Mortality percentage of female mosquitoes 24 h after exposure to the discriminating concentrations of the insecticides deltamethrin, etofenprox, and permethrin in WHO bioassays with impregnated paper. Mortality below 90% indicates resistance (dotted line). Populations are identified with the following abbreviations: SGC (São Gabriel da Cachoeira), BAR (Barcelos), MN (Manaus), TF (Tefé), CO (Coari), TAP (Tapauá), LB (Lábrea), ML (Mâncio Lima), RA (Rodrigues Alves), CZS (Cruzeiro do Sul), PV (Porto Velho), CJ (Candeias do Jamari), JAC (Jacareacanga), BG (Bagre), AN (Anajás), and PG (Porto Grande). Populations sharing the same color belong to the same state