Table 4 Summary of cost analysis for the 18s-rRNA nested and COX-I PCRs for Plasmodium spp. detection
PCR technique | Required PCR reagents | Estimated cost of the PCR kit | µL of polymerase used per reaction | Cost of PCR diagnosis per sample | Cost of 2122 reactions for Plasmodium detection |
|---|---|---|---|---|---|
18s-rRNA nested PCR | Taq polymerase kit (Invitrogen) | ~210 USD | 0.1 µL for nest-1 and 0.1 µL nest-2 | ~0.42 USD | ~892 USD |
Conventional COX-I single PCR | Taq polymerase kit (Invitrogen) | ~210 USD | 0.2 µL in a single reaction | ~0.42 USD | ~892 USD |
Fast COX-I single PCR | Blood Phusion polymerase kit (Thermo) | ~418 USD | 0.125 µL in a single reaction | ~0.26 USD | ~552 USD |