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Table 2 Summary of sequenced DNA samples (positive controls, Kenya and Solomon Islands) based on Plasmodium COX-I gene

From: Fast and robust single PCR for Plasmodium sporozoite detection in mosquitoes using the cytochrome oxidase I gene

Sample

Species IDa

E valueb

Coveragec (%)

Identityd

[GenBank identifier]e

PCR product from dilution 6 (0.043 pg of DNA)

 P. falciparum

P. falciparum

0

100

99.7%

[KM065500.1]

 P. vivax

P. vivax

1.3e–84

100

98.4%

[KF668441.1]

 P. knowlesi

P. knowlesi

8e–180

100

100%

[AB444108.1]

Known infective mosquitoes from the field (Kenya Highlands)

 Mosquitoes (n = 21)

P. falciparum

0

100

99.7%

[KM065500.1]

 Mosquitoes (n = 3)

P. ovale s.l.

1.21e–151

100

97.5%

[KF018660.1]

Positive mosquitoes from Western Province, Solomon Islands

 Mosquitoes (n = 17)

P. falciparum

0

100

99.7%

[KM065500.1]

 Mosquitoes (n = 5)

P. vivax

0

99

99.6

[KF668441.1]

 Mosquito (n = 1)

P. ovale wallikeri

7.1e–110

100

92.2%

[HQ712053.1]

  1. aBest BLASTed hit in GeneBank
  2. bProbability of observing the result by chance
  3. cPercentage coverage of entire sequence against best hit
  4. dPercentage of similarity against best hit
  5. eGeneBank ID of the best hit
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Malaria Journal

ISSN: 1475-2875

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